NanoStraw Technology

NanoStraw Transfection is Gentle, Repeatable and Cargo-Agnostic

 

 

The NanoStraw technology is a versatile platform technology, which establishes a direct, physical connection into cells to overcome a huge challenge faced by researchers globally: delivering materials into hard-to-transfect-cells in a gentle, non-perturbative manner.

 

Cargo delivery via NanoStraws drastically reduces the working steps, costs and technical difficulties involved in cell therapy manufacturing. By overcoming viability and manufacturing challenges plaguing traditional viral-based development, NAVAN is actively innovating therapies in a broad range of indications with strategic partnerships in place.

Intracellular delivery is a key step in investigating and engineering cells to treat a myriad of diseases

Stewart et al, NATURE research review, Oct. 2016

Novel Mechanism of Transport Enables Best-in-Class Characteristics

 

Cargo-Agnostic

Capable of delivering DNA, mRNA, and proteins with high efficiency, including functionally-active proteins such as Cas9.

 

 

Effectiveness of Delivery

Gentle, non-perturbative delivery is effective for a variety of primary cell types with high cell viability.

 

The uniform contact geometry also reduces the variability of the local voltage, so that a larger fraction of cells are porated at a particular voltage, with less cell death.

 

 

Dosage Control

NanoStraw platform allows for significantly tighter dosage distributions than standard chemical delivery.

 

 

Key features of NanoStraws that Enable Improved Dosage Control:

Ratio-metric control enables specific ratios of multiple components to be delivered simultaneously

 

Extremely fast (5 min.) expression in the cytoplasm due to lack of mRNA packaging or endocytosis

 

 

Fully-Scalable Approach

  • From a small number of cells (~5), relevant for rare cell types, to delivery of >100,000 cells at once in a single 96-well plate.

 

  • The upper limit on number of cells is simply a matter of the well size and thus can be easily increased or maintain multiple parallel wells for high throughput applications.