Our Approach

Nano-Injection Addresses Viral and Non-Viral Transfection Limitations

 

 

NanoStraws are born from conventional polymeric track-etched cell culture membranes. Membranes are coated with alumina and etched to produce fields of NanoStraws, of which the geometric parameters (diameter, thickness, and height) are fully controllable.

 

By piercing through membranes of seeded cells, NanoStraws provide a pipeline for the diffusion of biomolecules directly into the cytosol.

 

To ensure delivery of charged cargo such as DNA plasmids, RNA strands, and proteins into the cells, an electric field is applied to locally destabilize the lipid bilayer in contact with the NanoStraws. Very low pulses are utilized in this process, which mobilizes the cargo, driving it into the cell in a dosage-controlled fashion.

Interfacing Between the Cell Membrane and NanoStraws

Due to the close interface between the cell membrane and each NanoStraw, the electric field is localized, inducing transient membrane permeability only over a small area.

 

Short duration (20-200μs) electrical pulses with low voltage (about 20V) can serve as a valve to open the cell membrane and drive biomolecules into the cytoplasm.

 

Cellular engulfment of the NanoStraws significantly reduces the necessary electroporation voltage and increases homogeneity over a large area.